A urine pregnancy test detects the hormone hCG, which is produced shortly after a fertilized egg implants in the uterus. Using a urine sample, the test identifies hCG through a simple device—typically showing a line or symbol for a positive result. A negative result suggests no hCG is detected, though early testing or improper use may affect accuracy.
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First morning urine specimen is preferred since it generally contains the highest concentration of hCG; however, urine specimens collected at any time of the day may also be used.